• Molecular Biology/分子生物学
  • Plasmid DNA preparation/质粒制备
  • Subcloning/亚克隆
  • Custom ORF cloning/ORF克隆
  • Mutagenesis services/基因突变
  • Molecular diagnosis/分子诊断

• Protein Production Systems and Purification /蛋白质表达系统以及纯化

• The E. Coli System/大肠杆菌表达系统
  • Protein expression optimization and solubility test/蛋白质表达优化及可溶性表达筛选
  • Refolding/蛋白质折叠复性
  • Small scale expression and purification/小规模蛋白质表达及纯化
  • Scale up expression and purification/大规模蛋白质表达及纯化

• The Baculovirus System/昆虫细胞表达系统
  • Generation of high titer baculovirus stock (BV stock)/高低度病毒制备
  • Expression & optimization in Sf9, Sf21 and Hi5 cell lines/Sf9, Sf21 and Hi5细胞系表达优化
  • Pilot protein expression and purification/小规模蛋白质表达及纯化

• The Mammalian System/哺乳动物表达系统
  • CHO, Vero, BHK, 293 cells etc/CHO, Vero, BHK, HEK293 等多种表达系统
  • Adherence and suspension culture/贴壁及悬浮培养      
  • Transient expression screen/瞬转表达筛选
  • Stable cell line generation/稳定细胞系建立

• Crystallization & Structural Determination/蛋白质晶体结构测定
  • Crystallization condition screening/结晶条件筛选
  • Co-crystallization and soaking/蛋白质共结晶及小分子浸泡
  • 3-D structural determination /三维结构解析

• Computational Biology & Molecular Modeling/计算生物学和分子模型构建
  • Structural-Based Drug Design (SBDD)/基于结构的药物设计
    • Target family analysis: using prior knowledge (specificity)/目标族群分析：利用优先知识（特异性）
    • Homology modeling/同源建模
• Quantitative structure-activity relationship (QSAR)/定量构效关系
• Virtual drug screening/计算机虚拟筛选
  • Pharmacophore match for docking-based virtual screen/药物匹配对接的虚拟筛选
  • • Hypothesis-driven docking/假设驱动的对接
    • Enhancing results of virtual screens using data mining/提高使用数据挖掘的虚拟筛选的结果
    • De novo drug design/全新药物设计

详情请查看美迪西医药研发官网www.medicilon.com.cn