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| 信息标题：Carboxypeptidase B |
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信息类型：供应信息 |
一级类别：医药中间体 |
二级类别：生化试剂 |
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| 发布日期：2012-08-16 |
有 效 期：2222-12-01 |
| 产品价格：面议 |
| 产品规格：RC0201 |
| 包装说明：无 |
| 产品数量：100 |
| 点 击 数：1075 |
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| 【具体信息】 |
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RECOMBINANT CARBOXYPEPTIDASE B
RECOMBINANT
Expressed in E. Coli, sterile-filtered
SYNONYM
Peptidyl-L-lysine (L-arginine) hydrolase
ENZYME COMMISSION (EC) NUMBER:3.4.17.2
DESCRIPTIONS
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine carboxypeptidase B.
Expressed in E.Coli andpurified by chromatography.
No foreign enzyme activitysuch ascarboxypeptidaseA and chymotrypsin.
No protease inhibitors such as PMSF contain in preparation.
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine, lysine and ornithine. The enzyme is inhibited by metal chelating agents, e.g., EDTA.
ANALYSIS
Protein
Determined by Bradford method.
Unit Definition
One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
ADDITIVES
A solution in 100 mM sodium chloride and 20% glycerol in 10 mM Tris-HCl (pH=8.0).
ACTIVITY
≥100 units/mg protein
STABILITY
No activity loss within one year stored under −20°C
PACKAGING
Package size based on protein content.
STORAGE
-20℃
SAFETY
Contaminant
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine carboxypeptidase B.
Foreign activity
Otherproteases such as carboxypeptidase A, chymotrypsin, and trypsin, not detected.
Inhibitor
No protease inhibitors such as PMSF or EDTA contain in preparation.
PROCEDURE
For Carboxypeptidase B digestion of peptides, use a ratio (w:w) of about 1:100 to 1:1000 for Carboxypeptidase B:peptide.
REQUIRES SPECIAL SHIPPING: ICE PACK
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| 发布公司： 上海雅心生物技术有限公司 |
| 公司地址： 上海市松江区车墩工业区香闵路698号 |
| 邮 编： 201611 |
部 门： |
生产 |
| 联 系 人： 赵致 |
联系电话： |
021-37772412 |
| 手 机： 15901602030 |
传 真： |
021-37772413 |
| 企业商铺： 无 |
电子邮件： |
zhaozhi@yaxinbio.com |
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