|
|
| 信息标题：lipase A |
|
信息类型：供应信息 |
一级类别：医药中间体 |
二级类别：生化试剂 |
|
|
 |
|
| 发布日期：2012-08-16 |
有 效 期：2222-12-31 |
| 产品价格：面议 |
| 产品规格：RT0301 |
| 包装说明：无 |
| 产品数量：1000 |
| 点 击 数：1070 |
|
|
|
|
 |
| 【具体信息】 |
|
Recombinant lipase A （immobilized lipase A）
Enzyme Commission (EC) Number E.C.3.1.1.3
Recombinant lipase A produced by gene-technology;
Activity
200000 unit/g powder.
Descriptions
Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride.
Unit Definition
Lipase was assayed using p-nitrophenyl acetate ( pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute.
Additives
No other additives.
Packaging
Package size based on weight or activity content.
Application notes:
PH range of stability
pH5~10
Temperature range of stability
Be stable below 40°C, unstable at higher than 50°C.
Stability against organic solvents
Higher activity can be got in 10%-50% DMSO, isopropyl ether and petroleum ether. Higher activity can be got in 10%-25% ethanol, acetone and isopropanol, 50% activity can be kept in 50% acetone and isopropanol, no activity detected in 50% ethanol.
The recommended organics for usage of the immobilized lipase A:
Isopropyl ether and petroleum ether.
Application method
Used directly after suspended. And can be re-used more than 3 times (50% activity kept after 3 times usage)
Storage temp
Below 4°C. No activity loss within one year stored under -20°C
|
|
|
 |
| 发布公司： 上海雅心生物技术有限公司 |
| 公司地址： 上海市松江区车墩工业区香闵路698号 |
| 邮 编： 201611 |
部 门： |
生产 |
| 联 系 人： 赵致 |
联系电话： |
021-37772412 |
| 手 机： 15901602030 |
传 真： |
021-37772413 |
| 企业商铺： 无 |
电子邮件： |
zhaozhi@yaxinbio.com |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|  |
| 该公司发布的其它供应信息 |
|
|
|
|
|
|
|